Table 1. Scoring for muscle invasion at multiple tissue depths.
| Experimental group | 0 (No/little invasion) |
1 (Some invasion) |
2 (High invasion) |
p-value | |
|---|---|---|---|---|---|
| Individual 1 | Control | 5 | 8 | 5 | 0.04 |
| huCCL2si1 | 12 | 6 | 0 | ||
| huCCL2si2 | 11 | 7 | 0 | ||
| Individual 2 | Control | 0 | 7 | 11 | 0.0001 |
| huCCL2si1 | 12 | 4 | 2 | ||
| huCCL2si2 | 11 | 2 | 3 | ||
| Individual 3 | Control | 1 | 7 | 10 | 0.01 |
| huCCL2si1 | 9 | 6 | 3 | ||
| huCCL2si2 | 5 | 9 | 4 |
Three serial tumor sections at three different depths, 50 microns apart were scored in a blinded fashion by three different individuals using a numerical scoring system. 0= No to little invasion. 1= some invasion, 2= high invasion. The scores for each depth were averaged per sample, resulting in 3 scores per sample, and with n=6 tumor samples per group, this resulted in a total 18 scores per group. Statistical analysis was determined by Chi Square test. Statistical analysis was determined by p<0.05. Analyses by individuals are shown.