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. 2016 Jun 30;7(31):49368–49383. doi: 10.18632/oncotarget.10343

Figure 2. Transient Müller cells proliferation during retinal degeneration in RCS rats.

Figure 2

(AA1) Müller cells isolated from retinas of RCS-p+ rats and controls at postnatal day 8 and cultured in medium with growth factors (EGF and bFGF). There was no significant difference between the two groups in the clone formation efficiency of retinal progenitor cells. (BD2) DNA content analysis by flow cytometry after staining with propidium iodide. There was only a slight increase in the proportion of cells in the S phase in RCS-p+ rat retinas at p30 and p45 when compared with controls. Thereafter, the proportion of S phase cells in RCS-p+ retinas decreased. (EE6) Double-label immunofluorescence staining against BrdU (red) and CRALBP (green) shows that Müller cells proliferation was observed during the early stage of retinal degeneration, with the number of double positive cells gradually reducing after p30. Representative results are shown. Data are presented as the mean ± standard error from three replicates. *P < 0.05, **P < 0.01, Student's t-test.