Table 1. Antibodies used in immunofluorescence staining, Western blot and ISH.
| Antibody | Company | Con. | Speci | Cat# | Purpose |
|---|---|---|---|---|---|
| PAX6 | Santa Cruz Biotechnology | 1:200/1:1000 | Rabbit | sc-11357 | IF/WB |
| SOX2 | Abcam | 1:300/1:1000 | Rabbit | ab97959 | IF/WB |
| Lin28B | Cell Signaling | 1:1000 | Mouse | 5422S | WB |
| GS | Millipore | 1:600 | Mouse | Mab302 | |
| GFAP | Abcam | 1:800/1:1000 | Rabbit | ab48050-100 | IF/WB |
| Rhodopsin | Abcam | 1:300 | Mouse | ab81702 | IF |
| PKC-α | Abcam | 1:500 | Mouse | Ab32376 | IF |
| Brdu | Cell Signaling | 1:100 | Mouse | 5292S | IF |
| Anti-DIG-AP | Roche | 1:300 | Sheep | REF11093274910 | ISH |
| GAPDH | Cwbio, China | 1:2000 | rabbit | CW0101 | Internal control |
| β-actin | Cwbio, China | 1:1000 | mouse | CW0096 | Internal control |
| Secondary antibodies Cy3 (mouse) | Beyotime, China | 1:500 | goat | A0521 | IF |
| Secondary antibodies Cy3 (rabbit) | Beyotime, China | 1:500 | goat | A0516 | IF |
| Secondary antibodies Cy3 (goat) | Beyotime, China | 1:500 | donkey | A0502 | IF |
| Secondary antibodies Cy3 (mouse) | GeneTex | 1:500 | donkey | GTX85338 | IF |
| Secondary antibodies FITC (sheep) | Santa Cruz Biotechnology | 1:50 | donkey | SC-2467 | ISH |
| Secondary antibodies FITC (goat) | Santa Cruz Biotechnology | 1:200 | donkey | SC-2024 | IF |
| Secondary antibodies FITC (mouse) | Zhongshan goldenridge, China | 1:200 | goat | ZF-0312 | IF |
| Secondary antibodies Alexa-647 (sheep) | Jackson | 1:100 | donkey | 713-605-003 | IF |
| Secondary antibodies Alexa-647 (mouse) | Beyotime, China | 1:500 | goat | A0473 | IF |
| Secondary antibodies Alexa-647 (Rabbit) | Beyotime, China | 1:500 | goat | A0468 | IF |