Figure 1. ALDH1L1 is highly increased in NSCLC.

(A) Expression of ALDH1L1 in lung cancer cell lines was measured by multiple reaction monitoring mass spectrometry (MRM-MS). (B) Assessment of ALDH1L1 in IMR90 and H226 by immunofluorescence staining. Scale bar = 50 μm. (C) Representative immunohistochemical staining of ALDH1L1 in normal and cancerous lung tissue. Scale bar = 100 μm. Expression of ALDH1L1in cancerous (Cancer) and normal lung type I and II pneumocytes (Control). *p < 0.001, n = 57 for each case. (D) ALDH1L1 catalyses 10-formyltetrahydrofolate to THF with by-product of NADH in the serine-folate pathway. DHF, dihydrofolate; THF, tetrahydrofolate; SHMT1, serine hydroxymethyltransferase 1; DHFR, dihydrofolate reductase; CH₂-THF, 5,10-Methylenetetrahydrofolate; MTHFD1, methylenetetrahydrofolate dehydrogenase1.