Figure 2. Effect of ALDH1L1 over expression on induction of NADH and ATP production.

(A–C) EKVX and H23 cells were transfected with plasmid expressing ALDH1L1 for 24 h and incubated with siRNA of DHFR for 24 h. (D–F) EKVX and H23 cells were transfected with plasmid expressing DHFR for 24 h and incubated with siRNA of ALDH1L1 for 24 h. Effect of DHFR siRNA or ALDH1L1 siRNA on NADH/NAD+ (A, D) and ATP (B, E) was analysed. Immunoblot analysis was performed to confirm DHFR expression, ALDH1L1 knockdown (C, F). Data are representative of the mean and standard deviation three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 compared to vehicle control.