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. 2016 Jun 30;7(31):49397–49410. doi: 10.18632/oncotarget.10354

Figure 3. ALDH participates significantly in ATP synthesis using NADH through oxidative phosphorylation that requires the malate-aspartate shuttle in NSCLC.

Figure 3

(A–C) Effect of ALDH1L1 knockdown in the presence and absence of 10 mM malate on NADH/NAD+ (A) and ATP (B) was analysed. (D) Malate-aspartate shuttle for NADH transportation into the mitochondrial matrix. MAT, malate-α-ketoglutarate transporter; GAT, glutamate-aspartate transporter; OAA, oxaloacetate; α-KG, α-ketoglutarate. (E–G) Effect of GOT2 knockdown in the presence and absence of 10 mM malate on NADH/NAD+ (E) and ATP (F) was analysed. (H–J) Effect of MDH2 knockdown in the presence and absence of 10 mM malate on NADH/NAD+ (H) and ATP (I) was analysed. Immunoblot analysis was performed to confirm knockdowns of ALDH1L1, MDH2, GOT2 (C, G, J). Data are representative of the mean and standard deviation three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 compared to vehicle control.

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