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. 2016 Jun 30;7(31):49435–49449. doi: 10.18632/oncotarget.10356

Figure 4. ß-catenin inhibition decreases proliferation in human tumoral thyroid cells.

Figure 4

(A) FTC133 cells were infected with control (shScr) or ß-catenin (shßcat) lentivirus shRNAs. Total protein extracts were obtained at the indicated times and analyzed by western blot for ß-catenin expression. (B) Left panel: FTC133 cells were infected with control (shScr) or ß-catenin (shßcat) lentivirus shRNAs or a dominant-negative form of TCF4 [dnTCF4] and BrdU incorporation was calculated. Data show BrdU incorporation relative to that of cells infected with the vehicle vector (shScr). Values represent means ± SEM (n = 3). ***p < 0.001. Right panel: western blot analyis of TCF4 expression (C) FTC133 cells were infected with control (shScr) or ß-catenin (shßcat) lentivirus shRNAs and cell growth was estimated relative to the density at day 0. Values represent means ± SEM (n = 3). (D) Cell lines were infected with control (shScr) or ß-catenin (shßcat) lentivirus shRNAs and BrdU incorporation was calculated. Data show BrdU incorporation relative to that of the cells infected with the vehicle vector (shScr). ***p < 0.001.