Figure 1. EZH2 promotes glioma cell aerobic glycolysis.

(A–C) ECAR was measured by the Glycolysis Stress test in U87 and U251 glioma cell lines after the cells were treated with si-EZH2, Dznep, FH535, respectively. The glycolysis under basal conditions, the glycolytic capacity and the glycolytic reserve were analyzed according to the methods. *P < 0.05, **P < 0.01. Results are representative of at least three independent experiments. (D) U87 cells pretreated with lentivirus containing a luciferase reporter were implanted into the brains of nude mice, and tumor formation was assessed by bioluminescence imaging. Changes in bioluminescent signal were detected at day 7, 14, and 21 after implantation. Overall survival of nude mice was determined by Kaplan–Meier survival curves and log-rank test was used to assess the statistical significance of the differences.