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. 2016 Jul 1;7(31):49450–49458. doi: 10.18632/oncotarget.10370

Figure 4. EZH2 induces histone modifications and DNA methylation of miR-328.

Figure 4

(A) Correlation of EZH2 expression with miR-328 expression in 158 glioma tissues of CGGA data. (B) Level of miR-328 was analyzed in different glioma tissues of CGGA data. (C) Kaplan-Meier survival curves for miR-328 expression in GBM and 158 gliomas. (D) Western blot analysis of lysates from cells transfected by miR-328, probed with H3K27me3 antibody. GAPDH was served as the loading control. (E) Co-immunoprecipitation analyses of EZH2-H3 complex formation in U87 cells after Dznep treatment. (F) ChIP was performed on cell lysates using equal portions of anti-EZH2 as described in Materials and Methods. Input samples are DNAs amplified from lysates before immunoprecipitation. (G) Bisulfite sequencing of the miR-328 CpG island in glioma cells, Open and filled circles represent unmethylated and methylated CpG sites, respectively. Each horizontal row represents a single clone.