Skip to main content
. 2016 Jul 1;7(31):49459–49469. doi: 10.18632/oncotarget.10372

Figure 5. T cell skewing in CD1d knockout mice.

Figure 5

(A) CD1d−/− TCL1 mice generate CLL similar to CD1d+/+TCL1 mice. Depicted is a representative FACS stain from a CD1d−/− TCL1 mouse on splenocytes stained for CD3, CD19, CD5 and CD1d. On the middle and right FACS plot, CD5 and CD1d expression of gated CD3−CD19+ cells are depicted. (B) TCR-Vβ usage was determined in CD1d−/− (n = 4) and CD1d−/− TCL1 (n = 5) mice as described for Figure 1. (C, D) CD3+Vβ7+ T cells from CD1d−/− TCL1 mice were further stained for CD4 and CD8 expression and for NK1.1. Representative FACS profiles (C) and graphs (D) are shown. (DN: double negative for CD4 and CD8; iso: staining using an isotype control antibody instead of an anti-NK1.1 antibody). (Horizontal bars indicate mean percentage).