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. 2016 Jul 2;7(31):49527–49538. doi: 10.18632/oncotarget.10389

Figure 2. XL388 induces caspase-dependent apoptosis activation in OS cells-MG-63.

Figure 2

(A–C), U2OS (F) and SaOs-2 (F) OS cell lines, the primary human OS cells (line-1/−3) (G and H), as well as mouse osteoblastic MC3T3-E1 cells (F) and human OB-6 osteoblastic cells (G and H) were treated with applied concentration of XL388, cells were further cultured, and cell apoptosis was tested by listed assays. MG-63 cells, pretreated with z-VAD-fmk (vad, 25 μM), z-DVED-fmk (dved, 25 μM) or z-ITED-fmk (ited, 25 μM) for 1 hour, were stimulated with XL388 (100 nM), TUNEL staining assay (D) and colony formation assay (E) were performed. n = 5 for each assay. *p < 0.05 vs. group “C”. # p < 0.05 vs. XL388 only group (D–E).