Skip to main content
. 2016 Jul 2;7(31):49527–49538. doi: 10.18632/oncotarget.10389

Figure 3. XL388 inactivates mTORC1/2 in OS cells.

Figure 3

Listed OS cells were treated with applied concentration of XL388 for 8 hours, expressions of listed kinases (p- and regular) were tested by Western blots (A, C and D). Kinase phosphorylation was quantified (B). Stable MG-63 cells expressing T308A dominant negative AKT1 (“dnAKT1”) or empty vector (“Vec”, pcDNA3-puro) were treated with or without XL388, cells were further cultured, expressions of listed kinases were tested by Western blots (E), kinase phosphorylation was quantified (F); Cell survival and apoptosis were tested by MTT assay (G) and histone DNA ELISA assay (H), respectively. n = 3 for each assay. *p < 0.05 vs. group “C”. #p < 0.05 vs. “Vec” group (F–H).