Skip to main content
. 2016 Jul 2;7(31):49527–49538. doi: 10.18632/oncotarget.10389

Figure 5. XL388 activates autophagy in OS cells.

Figure 5

Listed OS cells were treated with applied concentration of XL388 for 24 hours, expression of listed proteins was tested by Western blots (A–C), quantification was also performed (A–C). MG-63 cells, pre-treated with 3-methyladenine (3-MA, 0.5 mM), chloroquine (Cq, 10 μM), or 0.1% of DMSO (“DMSO”) for 1 hour, were treated with XL388 (25/100 nM) for applied time, cell survival and apoptosis were tested (D and E). Stable MG-63 cells, expressing scramble shRNA (“sc shRNA”), Beclin-1 shRNA or ATG-5 shRNA (see Beclin-1/ATG-5 expression in (F), were treated with or without XL388 (25/100 nM) for applied time, cell survival and apoptosis were tested (G and H). n = 3 for each assay. *p < 0.05 vs. group “C”. #p < 0.05 vs. “DMSO” group (D–E). #p < 0.05 vs. “sc shRNA” group (G–H).