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. 2016 Jul 6;7(31):49636–49648. doi: 10.18632/oncotarget.10421

Figure 2. Metformin affects proliferation in H295R cell line.

Figure 2

Cell proliferation was evaluated by direct cell count and thymidine incorporation (4 hour pulse) in H295R cells treated in absence or presence of increasing concentrations of metformin (mM) at the indicated time points. Data are expressed as mean ± SE of cell count (A) or DNA-thymidine incorporation (B) percentage vs. non-stimulated controls in n =3 independent experiments. Metformin IC50s for cell count (C) and thymidine incorporation (D) were calculated on the dose-response curves obtained at the indicated time points. Statistical analysis was performed with ANOVA followed by Dunnett's post hoc test: §P < 0.001 vs. respective controls.