Figure 3. inhibition of peripheral estrogen synthesis does not prevent development of SSM2 tumor in bone.
(A) Representative H&E staining sections (original magnification, 10×. Scale bar = 100 μm. 60× magnification, scale bar = 25 μm) at 4 weeks post tumor cell inoculation. Presence of tumor cells is indicated by T. 105 SSM2 cells were injected into the right tibia of WT female mice (n = 3/ group), one week after SHAM- or OVX- surgery. In addition to OVX, one group (n = 3/group) received aromatase inhibitor (AI) for 4 weeks. Representative TRAP staining is shown (original magnification, 10×. Scale bar = 100 μm). As control, representative H&E and TRAP staining is shown on sections from tumor-free tibia. (B) Tumor grade score from 0 to 5 showing displacement of marrow by tumor, with 0 indicating no tumor and 5 100% tumor in the bone marrow. Data are represented as mean +/− sem. No statistical differences were observed between groups. (C) Number of osteoclasts (OC) per bone surface is shown for SHAM, OVX and OVX + AI groups. Data are represented as mean +/− sem. No statistical differences were observed between groups.