Figure 2. Characterization of nucleoside transporter activity implicated in Ara-C uptake.

(A) [³H]-cytidine uptake (1 μM, 1 min) by MV4-11, K562 and SEM cells and (B) [³H]-Ara-C transport (1 μM, 1min) in MV4-11 cell line were assayed. Cross-inhibitions were performed by adding to the transport medium cold guanosine (100 μM) and inhibition of hENT1 was achieved adding NBTI (1 μM) to the transport medium. Data are expressed as means ± SEM of triplicate measurements from three independent cultures.