Figure 2. WISP1 overexpression and knockdown by shRNA in CC cell lines.
(A) Real-time PCR and Western blot analysis identified significant increase in WISP1 expression in five CC cells and normal epithelial colon cells. (B, C) Real-time PCR and Western blot analysis identified significant decrease in WISP1 expression in LOVO and RKO cells treated with WISP1 shRNAs. (D) Real-time PCR and Western blot analysis identified significant increase in WISP1 expression in SW620 cells treated with pLVX-AcGFP-C1-WISP1 expressing vector (WISP1). shNC: pLVX-AcGFP-C1-scramble shRNA negative control. ***P < 0.001.