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. 2016 Jul 8;7(31):49834–49847. doi: 10.18632/oncotarget.10486

Figure 3. WISP1 shRNA inhibits cell proliferation by arresting cells at G1 phase.

Figure 3

LOVO, RKO and SW620 cells were infected with pLVX-AcGFP-C1-shWISP1 (shWISP1) or pLVX-AcGFP-C1-WISP1 expressing vector (WISP1) after 48 h. (A–C). Cells proliferation was detected by CCK-8 assay in LOVO, RKO and SW620 cells. (D) Cell cycle profile was analyzed using flow cytometry in LOVO and RKO cells. shNC: pLVX-AcGFP-C1-scramble shRNA negative control. NC: black pLVX-AcGFP-C1 negative control. *P < 0.05, ***P < 0.001.