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. 2016 Jul 8;7(31):49834–49847. doi: 10.18632/oncotarget.10486

Figure 8. Knockdown of WISP1 in CC cells reduces tumor growth in vivo.

Figure 8

LOVO cells infected with pLVX-AcGFP-C1-scramble shRNA negative control (shNC) or pLVX-AcGFP-C1-shWISP1 (shWISP1) were subcutaneously injected in athymic nude mice. (A) At day 45, mice were sacrificed and tumors were weighted. (B) Tumor growth was evaluated for 45 days. (C) Histology and PCNA expression were detected by HE staining and immunohistochemistry (IHC) assays. (D) Western blot analysis identified significant decrease in WISP1, β-catenin, ICAM-1, VCAW-1, VEGFC, MMP-2 and MMP-9, while increase in E-cadherin expression in LOVO cells infected with pLVX-AcGFP-C1-shWISP1 (shWISP1). shNC: pLVX-AcGFP-C1-scramble shRNA negative control. Scale bar: 10 mm. #P < 0.001, ***P < 0.001.