Figure 10. Extracellular vesicles derived from MDA-MB-231 cells promote fibroblast cell migration.

Nontransformed fibroblasts were plated on membrane with 8-micron pores in Transwell inserts with or without vesicles isolated from MDA-MB-231 cells as indicated, cultured in serum-free medium for 24 hours, and fixed with PFA. Cells on the upper side of the membranes were removed, and migrated cells on the lower side were stained with crystal violet, photographed, and counted. Cell motility was quantitated as the number of cells that migrated from the top of the membrane to the bottom of the membrane (mean+SEM, n=5). Double and triple asterisks indicate significant differences with p<0.01 and p<0.001, respectively, as indicated.