Figure 1. Stress-induced change of LSD1 and SIRT1 binding with KU70.

A. Co-IP of endogenous KU70 with SIRT1 (upper panel) or LSD1 (lower panel) at baseline and two time-points (24 and 48 hours) following 2.5μM Imatinib (IM) administration in KCL-22 cells. B. Co-IP of KU70 with LSD1 or SIRT1 without and with IM treatment for 24 hours in KU812 cells. C. Co-IP of KU70 with LSD1 or SIRT1 in KCL-22 cells treated with 0.25 μM CPT for 12 h or 1 mM H₂O₂ for 1 h. D. Co-IP of KU70 with LSD1 or SIRT1 in KCL-22 cells after γ-irradiation. Cell lysates were prepared 2 hours after radiation.