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A. Comet assay of KCL-22 and KU812 cells treated overnight with 0.5 μM CPT or 200 μM H2O2. CTL, control. Quantification of comet tail DNA was shown to the right. B. Left: real-time ChIP-PCR analysis of 8-Oxo-dG on ABL exons and β-actin after 2.5 μM IM treatment. Right: positive control of ChIP-PCR analysis of 8-Oxo-dG on ABL exon 6 and β-actin after 1 mM H2O2 treatment for 1 hour. * p<0.05; *** p<0.001.
