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. 2016 Jun 30;7(31):50195–50214. doi: 10.18632/oncotarget.10328

Figure 7. Opposing functions of LSD1 and SIRT1 in NHEJ repair.

Figure 7

A. Western blots of individual and combinatory knockdown of LSD1 and SIRT1. SCR, scrambled shRNA. B. NHEJ repair assay comparing individual and combinatory LSD1 and SIRT1 knockdown in KCL-22 cell-based NHEJ reporter cells. Bars represented mean percentage GFP positive cells with scrambled shRNA (shSP) or individual and combinatory gene knockdown. C. NHEJ reporter cells with SIRT1 or Scrambled (SCR) knockdown in the presence and absence of 50 or 200μM of the LSD1 inhibitor 2-PCPA. Dark gray bars represent percent of GFP cells without DOX induction, and light gray bars represent that with DOX induction. *P<0.01, **P<0.0001 and #P<0.01 when compared with SCR-DMSO; ♦P<0.01 compared with shSIRT1-DMSO. D. Left panel: immunoblots of NHEJ reporter cells transduced with vector alone, WT or K661A catalytically-inactive LSD1 expression vectors. HA-tagged LSD1 is detected using anti HA antibody. Right panel: comparing NHEJ repair with WT or K661A LSD1 over-expression. Bar graphs represented means (n=3) of percent GFP positive cells.