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. 2016 Jun 30;7(31):50195–50214. doi: 10.18632/oncotarget.10328

Figure 9. Opposing actions of LSD1 and SIRT1 in acquisition of HPRT mutations.

Figure 9

A. Spontaneous HPRT mutations in KCL-22 cells following LSD1 knockdown. 6-TG resistant colonies per million cells per well were scored, and normalized to the plating efficiency that was measured by soft agar colony numbers in the absence of 6-TG. B. Upper panel: HPRT mutations in prostate cancer PC3 cells upon individual and combinatory SIRT1/LSD1 knockdown. DNA damage was induced by 0.5μM camptothecin (CPT) treatment for 1 hour, and 15 million cells per plate were seeded for 6-TG selection. Plaques were scored and normalized to the plating efficiency (plaques in the absence of 6-TG) shown in the lower panel. P values were indicated as comparison to shSCR control in both A and B.