Figure 3. Slug and c-Jun were required for TMPRSS4-mediated invasion and proliferation.

PC3 cells were co-transfected with a TMPRSS4 expression vector or an empty vector and siRNA specific to Slug or c-Jun or negative control siRNA for 48 h. A. Transfected cells were allowed to invade Matrigel (8 × 10³ cells) for 48 h. The number of cells that had invaded was counted in five representative (×100) fields per Transwell insert. B. Transfected cells were seeded into 96-well plates at a density of 3000 cells/well and incubated for 48 or 72 h. Cell proliferation was determined by the colorimetric WST assay. Values represent mean ± SD. *P < 0.05 compared with empty vector + control siRNA; § P < 0.05 compared with TMPRSS4 + control siRNA. C. Transfected cells were lysed and used for immunoblotting. An anti-myc antibody was used to detect myc-tagged TMPRSS4. GAPDH was used as an internal control.