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. 2016 Jul 2;7(31):50349–50364. doi: 10.18632/oncotarget.10387

Figure 2. Expressions of neurotrophins and its receptors are modulated by YKL-40 expression level in cells.

Figure 2

A. Analyses of changes in mRNA expression were achieved on different neurotrophin receptors, TrkB, TrkC, p75NTR and co-receptor sortilin, NGF, BDNF and NT-3 by real time PCR in pLKO and sh YKL-40 cells. mRNA values represent fold-change (mean ± SD of three independent experiments) relative to mRNA levels corresponding to pLKO -transfected cells as controls (Student's t-test, *P< .05; **P< .01; *** P< .001). B. The pattern of protein expression from each gene was studied by western blotting in pLKO and sh YKL-40 cells. Values indicate the ratio of relative densitometric values of TrkB, TrkC, p75NTR, sortilin, NGF, BDNF and NT-3 compared to β-actin as loading control, normalized to an arbitrary value of 1 obtained with pLKO control cells. Results are representative of three independent experiments.