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. 2016 Jul 6;7(31):50392–50400. doi: 10.18632/oncotarget.10408

Table 2. Detailed primer sequence for Real-time PCR.

Primer Primer sequence Size/bp Tm/°C
Exon 5_Forward 5′-TCATTGCTTCTTGCTGATCTTGAC-3′ 74 59.8
Exon 5_Reverse 5′-GTGAGATTCTGAAGTTGAGCG-3′ 74 57
Exon 9_Forward 5′-GGTTCAACTACACGAATGGACCATGA-3′ 95 60
Exon 9_Reverse 5′-GTTCCCAGATGACTTGTCAGCCTTC-3′ 95 60.2
Exon 12_Forward 5′-GTGGACTGTGAAATGTATGGGCT-3′ 71 60.8
Exon 12_Reverse 5′-GCCATTCCAGCATATCGTCTTAG-3′ 71 59.3
Exon 16_Forward 5′-ATAGGATGTAATCAGACGACACAGGAA-3′ 120 58.5
Exon 16_Reverse 5′-CACTGCTGGAACTTCGCTCACA-3′ 120 59.4