Table 2. Detailed primer sequence for Real-time PCR.
Primer | Primer sequence | Size/bp | Tm/°C |
---|---|---|---|
Exon 5_Forward | 5′-TCATTGCTTCTTGCTGATCTTGAC-3′ | 74 | 59.8 |
Exon 5_Reverse | 5′-GTGAGATTCTGAAGTTGAGCG-3′ | 74 | 57 |
Exon 9_Forward | 5′-GGTTCAACTACACGAATGGACCATGA-3′ | 95 | 60 |
Exon 9_Reverse | 5′-GTTCCCAGATGACTTGTCAGCCTTC-3′ | 95 | 60.2 |
Exon 12_Forward | 5′-GTGGACTGTGAAATGTATGGGCT-3′ | 71 | 60.8 |
Exon 12_Reverse | 5′-GCCATTCCAGCATATCGTCTTAG-3′ | 71 | 59.3 |
Exon 16_Forward | 5′-ATAGGATGTAATCAGACGACACAGGAA-3′ | 120 | 58.5 |
Exon 16_Reverse | 5′-CACTGCTGGAACTTCGCTCACA-3′ | 120 | 59.4 |