Skip to main content
. 2016 Jul 6;7(31):50401–50416. doi: 10.18632/oncotarget.10409

Figure 1. LEF reduces cell viability and cell growth in RCC cells.

Figure 1

A. Cell viability was estimated by MST assay after Caki-2 and 786O cells were incubated with increasing concentrations of LEF for 48 h. DMSO was used as a control. B. The time-response curve of 200 μM LEF on cell viability of Caki-2 and 786O cells. Data in A and B represent mean ± SD from three independent experiments (*P<0.01, ** P<0.05, vs. the control). C. EdU incorporation assay was analyzed by fluorescence microscopy in Caki-2 cells treated with elevated concentrations of LEF (0-200 μM) for 48 h. Nuclei were visualized with Hoechst 33342. D. Representative images of cell colony formation assay to evaluate the long-term growth inhibition effects of LEF. Caki-2 cells were maintained in indicated concentrations of LEF for 7 days before staining with crystal violet.