Table 1. Primer sequences used for Real-time PCR.
Gene Name | Primer Sequences (5′→3′) |
---|---|
β-catenin | F: GCTTGTTCGTGCACATCAGGA R: TGTGAACATCCCGAGCTAGGA |
c-Myc | F: GGTGCTCCATGAGGAGACAC R: GCAGAAGGTGATCCAGACTC |
WNT1 | F: GAACTGTCCCACTGCTCCAG R: GGATTCGATGGAACCTTCTG |
WNT3a | F: ACTACGTGGAGATCATGCCC R: ATGAGCGTGTCACTGCAAAG |
WNT5a | F: TGAATAACCCTGTTCAGATGTCA R: TGTACTGCATGTGGTCCTGA |
WNT7a | F: CTGTGGCTGCGACAAAGAGAA R: GCCGTGGCACTTACATTCC |
WNT7b | F: GAAGCAGGGCTACTACAACCA R: CGGCCTCATTGTTATGCAGGT |
DKK1 | F: CCTTGAACTCGGTTCTCAATTCC R: CAATGGTCTGGTACTTATTCCCG |
FZD1 | F: GGGGCTTAACAACGTGGAC R: CAGAAAGGACGTGCCGATAAA |
FZD2 | F: GTGCCATCCTATCTCAGCTACA R: CTGCATGTCTACCAAGTACGTG |
FZD10 | F: GGCGGTGAAGACCATCCTG R: CAGCTTGTCCGTGTTCTCG |
GAPDH | F: CTCACCGGATGCACCAATGTT R: CGCGTTGCTCACAATGTTCAT |