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. 2016 Jul 8;7(31):50522–50534. doi: 10.18632/oncotarget.10494

Figure 6. SUMOylation of PES1 upregulates the transcriptional activities of ERα and its downstream target gene.

Figure 6

A. MCF-7 or T47D cells were transfected with ERE-Luc or with ERα, or with ERα and GFP-tagged wild-type PES1 or K517R. Luciferase activity was measured either with or without pre-treatment of the cells with 10 nM E2 for 16 h. B. Similar experiment as described in A was performed, but the cells were transfected with Cyclin D1-luciferase. C. MCF-7 or T47D cells were transfected with ERE-Luc or with ERα, or with ERα and GFP-tagged wild-type PES1 or K517R together with or without Flag-UBC9. Luciferase activity was measured either with or without pre-treatment of the cells with 10 nM E2 for 16 h. D. MCF-7 or T47D cells were transfected with wild-type PES1 or K517R. The cells were pre-treated with or without 10 nM E2 for 16 h and then subjected to RT-PCR to measure the mRNA level of Cyclin D1. The mRNA level of cyclin D1 was expressed relative to GAPDH transcriptional level. ‘*’, p<0.05; ‘**’, p<0.01.