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. 2016 Jul 8;7(31):50535–50547. doi: 10.18632/oncotarget.10495

Figure 2. HLA-A02 binding affinity and stability of the multipeptide cocktail and branched multipeptide.

Figure 2

a. Binding affinity: The multipeptide cocktail dose-dependently increased the HLA-A02 mean MFI in T2 cells. In cells treated with the branched multipeptide, the mean MFI increased until the total peptide concentration reached 22.5 μg/mL (7.5 μg/mL/peptide), which was selected as the optimal peptide concentration. b. Binding stability: While the stability of the branched multipeptides was maintained up to 24 hours post-BFA treatment, the stability of the multipeptide cocktail was only maintained up to 8 hours post-BFA treatment. The values represent the mean MFI ± SE of three separate experiments.