Table 1.
Details of qRT-PCR primers.
| Transcript | Primer name | Sequence (5′-3′ direction) |
|---|---|---|
| p53 | hu-p53-F | CGTCTGGGCTTCTTGCATTCT |
| hu-p53-R | AGCTGCACAGGGCAGGTCTT | |
| CDK4 | hu-CDK4-F | CTTCTGCAGTCCACATATGCAACA |
| hu-CDK4-R | CAACTGGTCGGCTTCAGAGTTTC | |
| CDK6 | hu-CDK6-F | TGGTGACCAGCAGCGGACAA |
| hu-CDK6-R | ACCACAGCGTGACGACCACT | |
| CDK2 | hu-CDK2-F | CCAGGAGTTACTTCTATGCCTGA |
| hu-CDK2-R | TTCATCCAGGGGAGGTACAAC | |
| p21Waf1 (CDKN1B) | hu-p21-F | GAGGAAGACCATGTGGACCTGT |
| hu-p21-R | GCGGATTAGGGCTTCCTCTTG | |
| P27KIP1 (CDKN1B) | hu-p27-F | GCAACCGACGATTCTTCTACTCAAA |
| hu-p27-R | GCTTCATCAAGCAGTGATGTATCT | |
| cyclin A2 | hu-CCNA2-F | GGGACAAAGCTGGCCTGAATC |
| hu-CCNA2-R | AGTGTCTCTGGTGGGTTGAGGA | |
| cyclin E1 | hu-CCNE1-F | AGTGTCTCTGGTGGGTTGAGGA |
| hu-CCNE1-R | GCTTGCACGTTGAGTTTGGGTA | |
| cyclin D1 | hu-CCND1-F | CCGTCCATGCGGAAGATC |
| hu-CCND1-R | CCTCCTCCTCGCACTTCTGT | |
| p150glued (DCTN1) | hu-DCTN-F | CACTTGTGATGAAGGGCATGG |
| hu-DCTN-R | ATCAGTTCCCTCTCTTTTGAGGAC | |
| EB1 (MAPRE1) | hu-MAPRE-F | CAGAGGCCCATCTCAACACA |
| hu-MAPRE-R | CAATACGTTGACCTGCTGCAT | |
| Ninein (NIN) | hu-NIN-F | ACTATATCCGGGACCGCCTT |
| hu-NIN-R | CGAGGTCACCAAACTTTTCTGC | |
| CEP150 | hu-CEP350-F | TAGCAGCCGCCAAGAAAGTC |
| hu-CEP350-R | GACGTTGAGTCTTTTTCATCAGGA | |
| RPS3 | hu-RPS3-F | AGCCACCAGAACACAGAATG |
| hu-RPS3-R | CTAGTGGCCACCTTTTCAGC |
Forward and reverse primers were designed to overlap atleast one exon-exon boundary of the target gene. The primer positions are based on the reference sequence of the target genes in the Genbank. Expression of ribosomal protein 3 gene (RPS3) or GAPDH (for CCNA2) was used as control for sample normalization in quantification of the gene of interest expression by real-time RT-PCR analysis.