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. 2017 Jan 11;12(1):e0169922. doi: 10.1371/journal.pone.0169922

Fig 1. Elevated IL-37 in TB patients and induction of IL-37 in response to Mtb infection.

Fig 1

(A) ELISA detection the abundance of IL-37 in the sera from healthy donors (HD) (n = 24) and TB patients (TB) (n = 24). Data are mean±SEM. Student’ t test was used for statistical analysis. (B) Western blot detection of IL-37 in the lysates of PBMC from HD (n = 7) and TB (n = 7). (C) Quantification and statistical analysis of the relative density (mean gray value) of IL-37/β-actin as shown in (B). Data are mean±SEM. Student’ t test was used for statistical analysis. (D) Induction of IL-37 transcripts measured by qRT-PCR in human THP-1 cells infected with H37Rv at MOI 5 for indicated time. (E) Secretion of IL-37 protein measured by ELISA in PMA-differentiated human THP-1 cells infected with H37Rv at indicated MOI for 24 h. Data (D-E) are mean±SEM and representative of 3 independent experiments. (F) Kinetics of IL-37 production measured by ELISA in the lung of WT and IL-37-Tg mice infected with BCG. Results are mean±SEM and are pooled from two independent experiments (n = 7 or 8 for each time point). *, p<0.05; **, p<0.01.