Fig 2. Immunoregulation is subject to a critical time window and IFNγ signalling in MSC is required for the inhibition of Vδ2+ proliferation.

(A) Analysis of intracellular IFNγ production in activated Vδ2+ cells at early time points. Results show the means ± S.D. of triplicate samples. *P ≤ 0.05. (B) Representative analysis of intracellular IFNγ production by Vδ2+ cells in the absence (upper panel) or presence (lower panel) of MSCs after 12h of co-culture. (C) Inhibition of proliferation of Vδ2+ cells by MSCs in a critical time window. Whole PBMCs were pre-activated for the indicated times and subsequently co-cultured with MSCs. Percentage of Vδ2+ cells were determined by Flow Cytometry five days after plating. Results show the means ± S.D. of triplicate samples.