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. 2017 Jan 11;12(1):e0169362. doi: 10.1371/journal.pone.0169362

Fig 3. Interfering with the IFNγ pathway in MSCs by specific silencing of the IFNγR and analysis of apoptosis in MSCs.

Fig 3

(A) Lentiviral-mediated shRNA transduction of MSCs was used to knock down the IFNγR. MSCs were transduced either with the empty vector (MSC-pLV) (upper panel) as control or with an IFNγR specific shRNA (MSC-IFNγRi) (lower panel). GFP expression in transduced cells was analysed four days after transduction by Flow Cytometry. (B) The efficiency of gene silencing was quantified by Real-Time qPCR in MSCs transduced either with the empty vector or with the one specific for IFNγRi. mRNA expression for IFNγR was significantly reduced in MSC-IFNγRi compared to MSC-pLV. Relative expression was normalized to the empty vector (MSC-pLV). (C) Transduction of MSCs does not lead to apoptotic cell death. Flow cytometric analysis of apoptotic cells (APC positive cells) in MSC-pLV (left) and MSC-IFNγRi (right) by AnnexinV staining 6 days after transduction.