Table 5.

Ratios of mutation types at the different target sites of five genes in mutant plants.

Target gene	Site	No. of examined lines	No. of lines with mutations	Deletion	Insertion	Substitution	Insertion and Substitution	Deletion and Substitution	Insertion, Deletion and Substitution	Mutation rate (%)	Average rate (%)
C3H	sgRNA1	10	0	0	0	0	0	0	0	0	16.7
	sgRNA2	10	1	0	0	1	0	0	0	0.1
	sgRNA3	10	4	0	1	3	0	0	0	0.4
C4H	sgRNA1	10	3	0	0	1	1	1	0	0.3	20
	sgRNA2	10	3	0	0	3	0	0	0	0.3
	sgRNA3	10	0	0	0	0	0	0	0	0
4CL	sgRNA1	10	0	0	0	0	0	0	0	0	33.3
	sgRNA2	10	6	1	0	5	0	0	0	0.6
	sgRNA3	10	4	0	4	0	0	0	0	0.4
CCR	sgRNA1	10	10	0	0	3	2	4	1	1	33.3
	sgRNA2	10	0	0	0	0	0	0	0	0
	sgRNA3	10	0	0	0	0	0	0	0	0
IRX	sgRNA1	10	0	0	0	0	0	0	0	0	6.7
	sgRNA2	10	2	0	0	2	0	0	0	0.2
	sgRNA3	10	0	0	0	0	0	0	0	0

The mutation rate is based on the number of mutation type lines out of the total number of all examined lines.