Table 1.

siRNA expression levels of transgenic p5001 TME 204 lines tested in confined field trials at Namulonge, Uganda, Mtwapa, coastal Kenya, and in a stake-derived trial at Namulonge, Uganda.

Line/Entry	Relative expression level (% of control)a	siRNA expressionb	Tested in Namulonge CFT	Tested in Mtwapa CFT	Tested in Stake-derived durability Trial
5001-01	66.9	+++	Yes	Yes	Yes
5001-08	97.3	+++	Yes	Yes	Yes
5001-09	100.3	+++	Yes	No	No
5001-10	99.6	+++	Yes	Yes	Yes
5001-16	7.8	+	Yes	Yes	No
5001-18	82.0	+++	Yes	Yes	Yes
5001-20	28.1	+	Yes	Yes	No
5001-22	56.0	++	Yes	No	No
5001-25	73.7	+++	Yes	No	No
5001-26	72.7	+++	Yes	Yes	Yes
5001-27	34.1	+	Yes	No	No
5001-30	79.7	+++	Yes	Yes	Yes
5001-34	99.3	+++	Yes	Yes	Yes
5001-35	102.5	+++	Yes	Yes	Yes
5001-40	19.9	+	Yes	Yes	No
5001-42	98.6	+++	Yes	Yes	No
5001-45	23.0	+	Yes	No	No
5001-46	78.7	+++	Yes	Yes	Yes
5001-47	79.0	+++	Yes	Yes	Yes
5001-48	10.6	+	Yes	No	No
5001-50	75.3	+++	Yes	Yes	Yes
5001-53	40.1	++	Yes	Yes	No
5001-69	76.7	+++	Yes	Yes	No
5001-76	45.5	++	Yes	Yes	No
5001-77	75.2	+++	Yes	Yes	No
718-01	100.0	+++	No	No	No

^asiRNA expression expressed relative to signal intensity of control line 718-01 (Ogwok et al., 2012) set at 100%.

^bRanking of transgenic lines based on siRNA expression levels (+ = low, 0–33.3% of control line 718-01 ++ = medium 33.4–66.6% of control line 718-01 and +++ high, 66.7–100% or more of control line 718-01).