Table 2.
CBSD incidence and severity in shoots and storage roots of transgenic p5001 TME 204 and controls plant lines at 6 and 12 months after planting (MAP) in a confined field trial conducted at Namulonge, Uganda.
| Line/entrya | Shoot | Storage roots (12 MAP) | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Av. severity score of CBSD symptoms (scale 1–5) | Number of plants harvested | Number of storage roots scored | Number. of storage roots showing CBSD symptomsb | Av. severity score of CBSD symptoms (scale 1–5) | Number of storage roots showing CBSD symptoms with score >2 | Usable/Marketable storage roots (%)c | ||||
| 6 MAP | 12 MAP | Count | (%) | Count | % | |||||
| 5001-01 | 1.0 | 1.0 | 45 | 251 | 7 | 2.8 | 2.6 | 6.0 | 2.4 | 97.6 |
| 5001-08 | 1.0 | 1.0 | 47 | 327 | 6 | 1.8 | 2.0 | 2.0 | 0.6 | 99.4 |
| 5001-09 | 1.0 | 1.0 | 47 | 226 | 78 | 34.5 | 2.7 | 17.0 | 7.6 | 92.4 |
| 5001-10 | 1.0 | 1.0 | 49 | 373 | 22 | 5.9 | 2.1 | 13.0 | 3.5 | 96.5 |
| 5001-16 | 2.8 | 3.2 | 45 | 264 | 230 | 87.1 | 3.8 | 220.0 | 83.3 | 16.7 |
| 5001-18 | 1.0 | 1.0 | 43 | 265 | 21 | 7.9 | 2.2 | 9.0 | 3.4 | 96.6 |
| 5001-20 | 3.0 | 3.4 | 43 | 217 | 171 | 78.8 | 3.7 | 164.0 | 76.3 | 23.7 |
| 5001-22 | 2.5 | 2.5 | 46 | 279 | 8 | 2.9 | 2.2 | 2.0 | 0.7 | 99.3 |
| 5001-25 | 1.0 | 1.0 | 35 | 185 | 1 | 0.5 | 2.0 | 0.0 | 0.0 | 100 |
| 5001-26 | 1.0 | 1.0 | 47 | 215 | 3 | 1.4 | 3.5 | 1.0 | 0.5 | 99.5 |
| 5001-27 | 2.9 | 2.2 | 41 | 214 | 116 | 54.2 | 3.4 | 116.0 | 54.0 | 46.0 |
| 5001-30 | 1.0 | 1.0 | 44 | 251 | 1 | 0.4 | 2.0 | 0.0 | 0.0 | 100 |
| 5001-34 | 1.0 | 1.0 | 49 | 293 | 16 | 5.5 | 2.2 | 2.0 | 0.7 | 99.3 |
| 5001-35 | 1.0 | 1.0 | 43 | 211 | 9 | 4.3 | 2.8 | 6.0 | 2.8 | 97.2 |
| 5001-40 | 3.4 | 3.6 | 50 | 260 | 239 | 91.9 | 4.2 | 232.0 | 88.9 | 11.1 |
| 5001-42 | 1.0 | 1.0 | 50 | 331 | 9 | 2.7 | 2.2 | 3.0 | 0.9 | 99.1 |
| 5001-45 | 2.8 | 3.1 | 47 | 199 | 107 | 53.8 | 4.3 | 103.0 | 51.5 | 48.5 |
| 5001-46 | 1.0 | 1.0 | 48 | 270 | 3 | 1.1 | 2.0 | 0.0 | 0.0 | 100 |
| 5001-47 | 1.0 | 1.0 | 46 | 259 | 10 | 3.9 | 2.0 | 1.0 | 0.4 | 99.6 |
| 5001-48 | 3.0 | 3.5 | 47 | 219 | 143 | 65.3 | 3.8 | 142.0 | 64.8 | 35.2 |
| 5001-50 | 1.0 | 1.0 | 49 | 243 | 4 | 1.6 | 2.2 | 1.0 | 0.4 | 99.6 |
| 5001-53 | 3.0 | 3.2 | 44 | 255 | 93 | 36.5 | 3.7 | 71.0 | 28.5 | 71.5 |
| 5001-69 | 1.0 | 1.0 | 48 | 276 | 19 | 6.9 | 2.5 | 2.0 | 0.8 | 99.2 |
| 5001-76 | 3.0 | 3.0 | 43 | 235 | 34 | 14.5 | 3.9 | 26.0 | 11.5 | 88.5 |
| 5001-77 | 1.0 | 1.0 | 41 | 228 | 10 | 4.4 | 2.2 | 4.0 | 1.8 | 98.2 |
| TME 204-WT1 | 2.6 | 3.2 | 49 | 265 | 264 | 99.6 | 4.2 | 200.0 | 79.1 | 20.9 |
| TME 204-WT3 | 2.8 | 3.4 | 50 | 277 | 263 | 94.9 | 4.5 | 209.0 | 75.5 | 24.5 |
| TME 204-WT3 | 2.9 | 3.4 | 44 | 219 | 219 | 100 | 4.4 | 222.0 | 100.0 | 0.0 |
| NASE 3 | 3.4 | 4.3 | 23 | 46 | 41 | 89.1 | 4.5 | 39.0 | 84.8 | 15.2 |
| NASE 14 | 4.1 | 4.4 | 29 | 89 | 78 | 87.6 | 4.0 | 72.0 | 80.0 | 20.0 |
MAP: months after planting
TME 204-WT1 and -WT2 represent entries of wild type TME 204 derived from in vitro micro-propagated plants. Entries TME 204-WT3, NASE 3, and NASE 14 wild-type plants were derived from disease-free stake cuttings obtained from farmers' fields.
All storage roots were sliced transversely into five roughly equivalent sized segments and each slice assessed for presence and severity of CBSD symptoms on a scale of 1–5 (Ogwok et al., 2012).
Usable/marketable storage roots were assessed as those showing disease severity score of 1–2 on their slices.