Table 4.
CBSD incidence and severity in shoots and storage roots of transgenic p5001 TME 204 and control plant lines at 6 and 12 months after planting (MAP) in a confined field trial conducted at Mtwapa, Kenya.
| Line/entrya | Shoot | Storage roots (12 MAP) | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CBSD incidence (%) | Av. severity score of CBSD symptoms (scale 1–5) | Number of plants harvest-ed | Number of storage roots scored | Number of storage roots showing CBSD symptomsb | Av. severity of CBSD symptoms (scale 1–5) | Number of storage roots showing CBSD symptoms with score >2 | Usable/Marketable storage roots (%)c | |||||
| 6 MAP | 12 MAP | 6 MAP | 12 MAP | Count | (%) | Count | % | |||||
| 5001-01 | 0.0 | 3.0 | 1.0 | 2.0 | 40 | 216 | 26 | 12.0 | 2.3 | 4.0 | 1.9 | 98.1 |
| 5001-08 | 0.0 | 0.0 | 1.0 | 1.0 | 40 | 251 | 32 | 12.7 | 2.0 | 1.0 | 0.4 | 99.6 |
| 5001-10 | 0.0 | 0.0 | 1.0 | 1.0 | 40 | 255 | 22 | 8.6 | 2.1 | 5.0 | 2.0 | 98 |
| 5001-16 | 0.0 | 3.0 | 1.0 | 2.0 | 40 | 259 | 28 | 10.8 | 2.2 | 10.0 | 3.9 | 96.1 |
| 5001-18 | 3.0 | 3.0 | 2.0 | 2.0 | 40 | 261 | 6 | 2.3 | 2.5 | 1.0 | 0.4 | 99.6 |
| 5001-20 | 5.0 | 5.0 | 2.0 | 2.0 | 40 | 223 | 20 | 9.0 | 2.6 | 10.0 | 4.5 | 95.5 |
| 5001-26 | 0.0 | 0.0 | 1.0 | 1.0 | 40 | 253 | 18 | 7.1 | 2.0 | 2.0 | 0.8 | 99.2 |
| 5001-30 | 0.0 | 0.0 | 1.0 | 1.0 | 40 | 241 | 20 | 8.3 | 2.0 | 0.0 | 0.0 | 100 |
| 5001-34 | 0.0 | 0.0 | 1.0 | 1.0 | 40 | 237 | 10 | 4.2 | 2.1 | 1.0 | 0.4 | 99.6 |
| 5001-35 | 0.0 | 0.0 | 1.0 | 1.0 | 40 | 239 | 11 | 4.6 | 2.0 | 0.0 | 0.0 | 100 |
| 5001-40 | 8.0 | 8.0 | 2.0 | 2.3 | 40 | 237 | 31 | 13.1 | 2.9 | 18.0 | 7.6 | 92.4 |
| 5001-42 | 3.0 | 5.0 | 2.0 | 2.0 | 40 | 234 | 13 | 5.6 | 2.1 | 4.0 | 1.7 | 98.3 |
| 5001-46 | 5.0 | 8.0 | 2.0 | 2.0 | 40 | 232 | 21 | 9.1 | 2.1 | 3.0 | 1.3 | 98.7 |
| 5001-47 | 0.0 | 5.0 | 2.0 | 2.0 | 40 | 229 | 8 | 3.5 | 2.1 | 2.0 | 0.9 | 99.1 |
| 5001-50 | 3.0 | 5.0 | 2.0 | 2.0 | 40 | 270 | 12 | 4.4 | 2.3 | 5.0 | 1.9 | 98.1 |
| 5001-53 | 0.0 | 0.0 | 1.0 | 1.0 | 40 | 240 | 13 | 5.4 | 2.1 | 1.0 | 0.4 | 99.6 |
| 5001-69 | 0.0 | 0.0 | 1.0 | 1.0 | 40 | 251 | 12 | 4.8 | 2.1 | 2.0 | 0.8 | 99.2 |
| 5001-76 | 0.0 | 3.0 | 1.0 | 3.0 | 40 | 248 | 15 | 6.0 | 2.1 | 1.0 | 0.4 | 99.6 |
| 5001-77 | 0.0 | 0.0 | 1.0 | 1.0 | 40 | 246 | 6 | 2.4 | 2.1 | 1.0 | 0.4 | 99.6 |
| TME 204-WT1 | 60.0 | 100.0 | 2.0 | 2.9 | 40 | 253 | 242 | 95.7 | 4.0 | 226.0 | 89.3 | 10.7 |
| TME 204-WT2 | 40.0 | 100.0 | 2.0 | 3.0 | 40 | 280 | 243 | 86.8 | 3.6 | 214.0 | 76.4 | 23.6 |
MAP: months after planting.
a
TME 204-WT1, and -WT2 represent entries of wild-type TME 204 derived from tissue culture micro-propagated plants.
b
All storage roots were sliced transversely into five roughly equivalent sized segments and each slice assessed for presence and severity of CBSD symptoms on a scale of 1–5 (Ogwok et al., 2012).
c
Usable/marketable storage roots were assessed as those showing disease severity score of 1–2 on their slices.