FIGURE 7.

Toll-like receptors/dsRNA complex inhibitor can decrease the viral loads of IAV in P815 cells. P815 cells treatment with TLR3/dsRNA complex inhibitor were infected with H1N1, H5N1, and H7N2 at a MOI of 0.1, exposed to LE-PolyI:C, or mock treated. Cells were homogenized in Trizol and relative viral NS gene quantification was determined by real time PCR (A). Culture supernatants were collected at the indicated times post-infection, and virus titers in the supernatants were determined by plaque assay (B). Results shown are pooled from three independent repeats. Asterisks indicate statistically significant increases compared to mock treatment (^∗P < 0.05, ^∗∗P < 0.01).