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. 2017 Jan 12;9:168. doi: 10.3389/fnmol.2016.00168

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Copyright © 2017 Parker, Welch, Forster, Tasneem, Dubhashi and Baro.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Mouse HCN2 is SUMOylated in vivo. Mouse forebrain non-denatured (A) and denatured (B) membrane fractions were used in immunoprecipitation (IP) experiments with an antibody against HCN2 or IgG (negative control). Western blots (WBs) containing the IP products were probed for HCN2, SUMO1, and SUMO2/3. The experiment was repeated three times using the brains from three different mice. Representative WBs are shown from a single experiment. The red dots indicate non-specific products pulled down in the IP. The single band at ∼120 kDa represents HCN2 channels. The asterisk indicates a non-covalently bound, SUMOylated protein. The band at ∼50 kDa represents the IP antibody.