FIGURE 10.

Enhanced SUMOylation at K669 increases GFP-HCN2 channel surface expression. The K669R mutant cell line was transiently transfected with mCherry or mCherry + SUMO + Ubc9, followed by the biotinylation assay to measure GFP-HCN2 surface expression. (A) Representative blots probed with anti-GFP, anti-Na/K ATPase, and anti-actin antibodies. (B) Plots of normalized GFP-HCN2 channels in each treatment group. The treatment and the n are shown below the graph. Each n represents a single plate that was transfected, biotinylated, and carried through the experiment to produce a single lane on a WB. There was no significant difference between treatment groups (Student’s t-test, p = 0.5606).