Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jan 12;9:168. doi: 10.3389/fnmol.2016.00168

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2017 Parker, Welch, Forster, Tasneem, Dubhashi and Baro.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

GFP-HCN2 channels are SUMOylated in Hek-HCN2 cells. Cell lysates from the Hek-HCN2 cell line were used in IP experiments with an antibody against GFP. WBs containing the IP products were then probed with an anti-GFP antibody or an anti-SUMO2/3 antibody. Parental Hek cell lysates were also probed with the anti-SUMO2/3 antibody. The anti-SUMO2/3 antibody recognized the GFP-HCN2 doublet in the Hek-HCN2 cell line, but not in the parental cell line, suggesting that GFP-HCN2 channels are SUMOylated.