Table 1.
Primary antibodies.
| Antigen | Verification of specificity | Manufacturer, species, cat no. | Concentration used |
|---|---|---|---|
| SUMO2/3 | Specificity verified by company through Western blot (WB) analysis of Hek-293 cells transfected with SUMO2 | Santa Cruz Biotechnology, rabbit polyclonal, cat. no. sc-32873 | WB: 1:3000 |
| SUMO1 | Specificity verified by company through WB analysis of Hek-293 with and without SUMO1 expressed | Santa Cruz Biotechnology, rabbit polyclonal, sc-9060 | WB: 1:3000 |
| GFP (for WB) | Specificity verified by company through WB analysis of cells with and without GFP expressed | Santa Cruz Biotechnology, rabbit polyclonal, cat. no. sc-8334 | WB: 1:4000 |
| GFP (for IP) | We verified specificity through WB analysis (Figure 2) | Abcam, rabbit polyclonal, cat. no. ab290 | 1 μl per 500 μg of lysate |
| HCN2 | Specificity verified by company through WB analysis of cells with and without HCN2 expressed | Santa Cruz Biotechnology, goat polyclonal, sc-19708 | WB: 1:3000 (Hek cells) |
| HCN2 | Specificity verified by company through WB analysis of mouse and rat membrane proteins | UC Davis/NIH NeuroMab, mouse monoclonal, N71/37 | WB 1:200 (Mouse brain) |
| Actin | Specificity verified by company through WB analysis of non-transfected and β-Actin transfected Hek-293 cells | Santa Cruz Biotechnology, rabbit polyclonal, sc-1616-R | WB: 1:2000 |
| Na+/K+-ATPase | Specificity verified by company through WB analysis of Human, Mouse and Rat tissues expressing the Na+/K+-ATPase | Abcam, mouse monoclonal, cat. no. ab7671 | WB: 1:3000 |