Table 2.

Site directed mutagenesis primers.

Primer name	Primer sequence
mHCN2K534RFor	5′-CACAGCCATGCTGACAAAGCTCAg ATTTGAGGTCTTCCAGCCTGG-3’
mHCN2K534RRev	5′-CCAGGCTGGAAGACCTCAAATcTGAGCTTT GTCAGCATGGCTGTG-3’
mHCN2K669RFor	5′-GCCATCATCCAGGAGATTGTCAgATATGACCG TGAGATGGTGCAGC-3’
mHCN2K669RRev	5′-GCTGCACCATCTCACGGTCATATcTGACA ATCTCCTGGATGATGGC-3’