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. 2017 Jan 12;7:667. doi: 10.3389/fimmu.2016.00667

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Copyright © 2017 White, Beaver, Goodier, Bottomley, Nielsen, Wolf, Boldrin, Whitmore, Morgan, Pearce and Riley.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Natural killer (NK) cell maturation status only partially explains altered LY49R expression in calorie-restricted (CR) mice. Characterization of C57BL/6 murine splenic NK cells by maturation status (numbered 1 through 4 below the x-axis, as defined in Figure 5A) in control (shaded boxes) and CR mice (open boxes) at 6 months (6 MO), 12 months (12 MO), and 22 months (22 MO). (A–E) Plots represent the percentage NK cells (NKp46⁺, CD3⁻) in each CD27/CD11b defined subset expressing (A) Ly49H, (B) Ly49F, (C) Ly49A, (D) Ly49D, and (E) Ly49G2. For gating, see Figure S4 in Supplementary Material. Horizontal bars represent median values, boxes extend from the 25th to the 75th percentile, and whiskers represent the min to max range. p Values are derived from non-parametric Mann Whitney U tests. ****p ≤ 0.0001, ***p < 0.001, **p < 0.01, and *p < 0.05. Numbers of mice per group ranged from 10 to 13.