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. 2017 Jan 6;8:13824. doi: 10.1038/ncomms13824

Figure 4. BMP activation has no direct inhibitory effects on Wnt signalling in Lgr5+ ISCs.

Figure 4

(a) Heatmap showing the expression levels of several canonical Wnt target genes in Lgr5+ stem cells on BMP stimulation. (b) Quantitative RT–PCR verification of BMP-regulated genes and Wnt target genes in Lgr5+ stem cells. Data represent mean±s.e.m of n=3 independent experiments. ***P<0.001 by Student's t-tests. (c) Immunoblots of cytoplasmic and nuclear proteins from intestinal organoids treated with or without BMP4 for 4 h. Data are representative of three independent experiments. (d) Quantitative RT–PCR analysis of gene expression in Apc mutant organoids derived from intestinal tumours in Vil-cre;Apcwt/fl mice that were treated with or without BMP for 4 h. Data represent mean±s.e.m of three independent experiments. *P<0.05, **P<0.01, ***P<0.001 by Student's t-tests. Scale bar, 50 μm.