Fig. 1. TBMS1 downregulates the expression of CXCR4 in breast cancer cells. (A) MDA-MB-231 cells or (B) T47D, MDA-MB-435 and MCF-7 cells were treated with TBMS1 for 24 hr at the indicated concentrations, and the expression of CXCR4, CXCR7 and β-actin were analyzed by immunoblotting. (C) MDA-MB-231 cells or (D) T47D, MDA-MB-435 and MCF-7 cells were treated with TBMS1 for 24 hr. The mRNA expression of CXCR4, CXCR7 and GAPDH were either examined by reverse-transcript PCR or (E) real-time PCR, as indicated. **P < 0.01 compared with DMSO treated cells. Representative results of three independent experiments were shown.
