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. 2016 Dec 29;2016:7053872. doi: 10.1155/2016/7053872

Figure 3.

Figure 3

Expression of E-cadherin (a), MUC2 (b), vimentin (c), and α-SMA (d) in human ALI tumor colorectal organoids. Comparison of a cancer stem cell marker, LGR5 expression in tumor organoids (e), and primary tumor tissues (f). The number of LGR5-positive cells in the pictures was counted ((g) n = 4, each for three patients (T1, T2, and T3)). ∗ P < 0.05 versus organoid. Expression of CD44 (h) in human ALI tumor colorectal organoids. The paraffin sections (4 μm) were immunofluorescently stained using antibody against E-cadherin, MUC2, vimentin, α-SMA, LGR5, and CD44. Representative photomicrographs are shown (n = 4). Culture days 14–21. Scale bar: 50 μm.