Skip to main content
. 2016 Dec;8(12):3682–3690. doi: 10.21037/jtd.2016.12.22

Figure 4.

Figure 4

LMO4 knockdown inhibits non-small-cell lung cancer (NSCLC) cell migration. (A) Protein expression of LMO4, c-met, MPAK, AKT, and PI3K were analyzed by western blots in A549 and H1299 cells; (B) LMO4, p-AKT, p-PI3K protein levels were up regulated in A549 and H1299 cells transfected with LMO4, as detected by immunofluorescence (magnification, ×600); (C) the Boyden assay showed that LMO4 increased NSCLC cell invasion, an effect abrogated by LY294002 (magnification, ×20). Error bars represent mean ± SD from three independent experiments. *, P<0.05; **, P<0.05; (D) wound healing assays of A549 and H1299 cells transfected with control vectors, LMO4, and LMO4 combined with LY294002 at 0 and 48 h after wounding (magnification, ×20).